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By doing this you are supporting our transition to become a non riding venue is so valuable to us and the welfare of our elephants. We are a privately owned charity run by one family trying to improve the situation for domestic elephants throughout the country. we rely on the money from tourism to care for 8 resident elephants most of whom have been taken from worse off situations and also the money funds our mobile veterinary unit which treats sick elephants all over Sri LankaSo many thanks for your contribution upon entranceReviewed August 19, 2016 Great experience close up with elephantsA great experience walking with an elephant.

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New Delhi metallo lactamase (NDM) represents a serious challenge for treatment and public health. A carbapenem resistant Escherichia coli clinical strain WCHEC13 8 was subjected to antimicrobial susceptibility tests, whole genome sequencing and conjugation experiments. It was resistant to imipenem (MIC, >256 g/ml) and meropenem (MIC, 128 g/ml) and belonged to ST3835. blaNDM 1 was the only carbapenemase gene detected. Strain WCHEC13 8 also had a plasmid borne AmpC gene (blaCMY 42) and two extended spectrum lactamase genes (blaCTX M 15 and blaSHV 12). blaNDM 1 and blaSHV 12 were carried by a 54 kb IncX3 self transmissible plasmid, which is identical to plasmid pNDM HF727 from Enterobacter cloacae. blaCMY 42 was carried by a 64 kb IncI1 plasmid and blaCTX M 15 was located on a 141 kb plasmid with multiple F replicons (replicon type: F36:A4:B1). blaCMY 42 was in a complicated context and the mobilisation of blaCMY 42 was due to the transposition of IS Ecp1 by misidentifying its right end boundary. Genetic context of blaNDM 1 in strain WCHEC13 8 was closely related to those on IncX3 plasmids in various Enterobacteriaceae species in China. In conclusion, a multidrug resistant ST3835 E. coli clinical strain carrying blaNDM 1, blaCTX M 15, blaCMY 42 and blaSHV 12 was identified. IncX3 plasmids may be making a significant contribution to the dissemination of blaNDM among Enterobacteriaceae in China.



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